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ehei  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ehei
    Ehei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ehei/pm31529521-222-14-20
    Average 90 stars, based on 1 article reviews
    ehei - by Bioz Stars, 2026-10
    90/100 stars

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    Polymerase Chain Reaction:

    Article Title: Structural Basis for the Enzymatic Formation of the Key Strawberry Flavor Compound 4-Hydroxy-2,5-dimethyl-3(2H)-furanone
    Article Snippet: Cloning, Expression, and Purification of FaEO—FaEO was amplified via polymerase chain reaction (PCR) from pET29aFaQR (16) usingPfuDNApolymerase (Fermentas/ThermoScientific, St. Leon-Rot, Germany) and the phosphorothioate primers (18) 5 -GCC GCA GCT CCA AGT GAG TCC-3 und 5 -GTG GTG CCA TGG TTA GAT GGG ATA CAC AAC CAC C-3 , generating a 5 blunt end compatible with EheI and a 3 sticky end compatible with NcoI (both underlined). .. The PCR product was cut with NcoI (New England Biolabs, Ipswich, MA) and ligated with T4 DNA ligase (Fermentas/ Thermo Scientific) with the vector pASK-IBA5plus (IBA, Göttingen, Germany), which had been cut with EheI (Fermentas/ Thermo Scientific) and NcoI. .. The resulting plasmid, pASKStrep-FaEO was analyzed by restriction digest and DNA sequencing (ABI Prism 310; Applied Biosystems/Invitrogen).

    Article Title: Structural Basis for the Enzymatic Formation of the Key Strawberry Flavor Compound 4-Hydroxy-2,5-dimethyl-3(2 H )-furanone
    Article Snippet: FaEO was amplified via polymerase chain reaction (PCR) from pET29a-FaQR ( 16 ) using Pfu DNA polymerase (Fermentas/Thermo Scientific, St. Leon-Rot, Germany) and the phosphorothioate primers ( 18 ) 5′- GCC GCA GCT CCA AGT GAG TCC-3′ und 5′-GTG GTG CCA TGG TTA GAT GGG ATA CAC AAC CAC C-3′, generating a 5′ blunt end compatible with EheI and a 3′ sticky end compatible with NcoI (both underlined). .. The PCR product was cut with NcoI (New England Biolabs, Ipswich, MA) and ligated with T4 DNA ligase (Fermentas/Thermo Scientific) with the vector pASK-IBA5plus (IBA, Göttingen, Germany), which had been cut with EheI (Fermentas/Thermo Scientific) and NcoI. .. The resulting plasmid, pASK- Strep -FaEO was analyzed by restriction digest and DNA sequencing (ABI Prism 310; Applied Biosystems/Invitrogen).

    Purification:

    Article Title: Nucleic acid constructs for genome editing
    Article Snippet: .. DNA samples were analyzed for indel mutations using the restriction enzyme site loss method (Marton et al., 2010) Briefly, purified N. benthamiana DNA samples were first digested by EheI (Fermentas) for NptII target or MlyI (SchI) (Fermentas) for PDS target. ..



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    Summary of the restriction analysis of phage S144 DNA, propagated on C. sakazakii CS1, S. Infantis S15 and S. Muenster S394. DNA from phage Lambda (methylated and unmethylated) have been used as controls. The restriction site for each of the nine used enzymes is specified and in each cell, it is indicated if the DNA was cut (1, in orange) or not (0, in white). SspI and PacI, the only two enzymes able to cut S144 DNA, are in bold. RE, restriction enzymes; RS, restriction sites, met., methylated; unmet., unmethylated.
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    Summary of the restriction analysis of phage S144 DNA, propagated on C. sakazakii CS1, S. Infantis S15 and S. Muenster S394. DNA from phage Lambda (methylated and unmethylated) have been used as controls. The restriction site for each of the nine used enzymes is specified and in each cell, it is indicated if the DNA was cut (1, in orange) or not (0, in white). SspI and PacI, the only two enzymes able to cut S144 DNA, are in bold. RE, restriction enzymes; RS, restriction sites, met., methylated; unmet., unmethylated.

    Journal: International Journal of Molecular Sciences

    Article Title: Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

    doi: 10.3390/ijms21155196

    Figure Lengend Snippet: Summary of the restriction analysis of phage S144 DNA, propagated on C. sakazakii CS1, S. Infantis S15 and S. Muenster S394. DNA from phage Lambda (methylated and unmethylated) have been used as controls. The restriction site for each of the nine used enzymes is specified and in each cell, it is indicated if the DNA was cut (1, in orange) or not (0, in white). SspI and PacI, the only two enzymes able to cut S144 DNA, are in bold. RE, restriction enzymes; RS, restriction sites, met., methylated; unmet., unmethylated.

    Article Snippet: S144 DNA was digested with the FastDigest restriction enzymes EheI, MauBI, PacI, Cfr42I, XhoI, NotI, Bsp120I, SspI and SspDI (respectively, FD0443, FD2084, FD2204, ER0201, FD0694, FD0596, FD0134, FD0774, FD0774 and ER2191 from Thermo Fisher).

    Techniques: Methylation